Cell line | Transfected | Endogenous HLA A alleles | Total # of cells (million) | Enriched by W6/32 antibody | Enriched by isotype control antibody | Predicted binding peptide by NetMHC 4.0 | Predicted binding affinity by NetMHC 4.0 (unit: nmol/L, the lower value the stronger binding) | Detected abundance (femtomole) | Detected copy number per cell |
---|---|---|---|---|---|---|---|---|---|

COS-7 | HLA-A3 + KRAS G12C vector | N/A | 285.3 | YES | NO | VVGACGVGK (9-mer) | 221.43 | N/D | N/D |

N/A | VVVGACGVGK (10-mer) | 375.53 | N/D | N/D | |||||

COS-7 | HLA-A3 + KRAS G12D vector | N/A | 270.9 | YES | NO | VVGADGVGK (9-mer) | 1,172.08 | 64 ± 6.3 | 196 ± 19.3 |

N/A | VVVGADGVGK (10-mer) | 938.8 | 6 ± 0.53 | 21 ± 1.9 | |||||

COS-7 | HLA-A3 + KRAS G13D vector | N/A | 286.7 | YES | NO | VVGAGDVGK (9-mer) | 1,552.04 | 26 ± 3.7 | 75 ± 10.7 |

N/A | VVVGAGDVGK (10-mer) | 936.84 | 2.2 ± 0.5 | 7 ± 1.6 | |||||

COS-7 | HLA-A2 + KRAS G12C vector | N/A | 261.9 | YES | NO | N/A | N/A | N/D | N/D |

COS-7 | HLA-A2 + KRAS G12D vector | N/A | 300.3 | YES | NO | N/A | N/A | N/D | N/D |

COS-7 | HLA-A2 + KRAS G13D vector | N/A | 276.6 | YES | NO | N/A | N/A | N/D | N/D |

COS-7 | HLA-A1 + KRAS WT vector | N/A | 290.2 | YES | NO | ILDTAGQEEY | 86.69 | N/D | N/D |

COS-7 | HLA-A1 + KRAS Q61H vector | N/A | 254.9 | YES | NO | ILDTAGHEEY | 185.91 | 178 ± 12.2 | 583 ± 40.0 |

COS-7 | HLA-A2 + KRAS Q61H vector | N/A | 266.2 | YES | NO | N/A | N/A | N/D | N/D |

COS-7 | HLA-A1 + KRAS Q61L vector | N/A | 267.6 | YES | NO | ILDTAGLEEY | 66.03 | 136 ± 13.5 | 512 ± 50.8 |

COS-7 | HLA-A2 + KRAS Q61L vector | N/A | 269.6 | YES | NO | N/A | N/A | N/D | N/D |

COS-7 | HLA-A1 + KRAS Q61R vector | N/A | 261.9 | YES | NO | ILDTAGREEY | 322.23 | 40 ± 3.5 | 127 ± 11.1 |

COS-7 | HLA-B7 + IDH2 R140Q vector | N/A | 273.3 | YES | NO | SPNGTIQNIL | 228.61 | 8 ± 1.3 | 25 ± 4.1 |

COS-7 | HLA-B7 + IDH2 R140Q vector | N/A | 289.2 | NO | YES | N/A | N/A | N/D | N/D |

COS-7 | HLA-A3 + IDH2 R140Q vector | N/A | 268.6 | YES | NO | N/A | N/A | N/D | N/D |

RPMI-6666 | Not transfected | A*02:01, A*03:01 | 2,100 | YES | NO | N/A | N/A | N/D | N/D |

HH | Not transfected | A*01:01, A*01:01 | 2,000.0 | YES | NO | N/A | N/A | N/D | N/D |

CFPAC-1_G12D knock-in | Not transfected | A*02:01, A*03:01 | 83.1 | YES | NO | VVGADGVGK (9-mer) | 1,172.08 | 4 ± 0.5 | 40 ± 5.0 |

HS578.T | Not transfected | A*03:01, A*24:02 | 70.4 | YES | NO | VVGADGVGK (9-mer) | 1,172.08 | 6 ± 0.6 | 71 ± 7.1 |

HL-60 | Not transfected | A*01:01, A*01:01 | 1,990.1 | YES | NO | ILDTAGLEEY | 66.03 | 10 ± 1.0 | 4 ± 0.4 |

SW948 | Not transfected | A*01:01, A*01:01 | 326.1 | YES | NO | ILDTAGLEEY | 66.03 | 6 ± 0.7 | 15 ± 1.75 |

RD | Not transfected | A*01:01, A*01:01 | 412.9 | YES | NO | ILDTAGHEEY | 185.91 | 4 ± 0.3 | 8 ± 0.6 |

Hs940.T | Not transfected | A*01:01, A*01:01 | 131.3 | YES | NO | ILDTAGREEY | 322.23 | 0.2 ± 0.02 | 1 ± 0.1 |

Abbreviations: N/A, not available; N/D, not detectable.