Table 4

Normal tissue expressiona profiles of CT genes as determined by RT-PCRb.

CT Genes with Testis-Restricted mRNA Expression (µg cDNA product)
BAGE/CT2.1 (0.02), MAGEE1/ CT10 (>2.0), CSAGE/CT24.1 (0.01), SPO11/CT35 (0.02), MAGEB1/CT3.1 (>2.0), SAGE/CT14 (0.02), MMA-1/CT25.1a (0.02), TPX1/CT36 (0.02), GAGE1/CT4.1 (>2.0), LIP1/CT17 (0.02), HOM-TES-85/CT28 (0.75), FTHL17/CT38 (>2.0), SSX2/CT5.2 (0.04), NA88/CT18 (0.01), LDHC/CT32 (0.04), TPTE/CT44 (0.08), BRDT/CT9 (0.02), CTAGE-1/CT21.1 (0.02), MORC/CT33 (0.06)
CT Transcripts Detected in 2 or Fewer Non-gametogeic Tissues
CT Transcript Tissue (µg cDNA product)
MAGEA1/CT1.1 testis (>2.0), pancreas (0.02)
NY-ESO-1/CT6.1 testis (>2.0), placenta (0.05), pancreas (0.02), liver (0.11)
MAGEC1/CT7.1 testis (0.14), pancreas (0.09)
SYCP1/CT8 testis (>2.0), pancreas (0.03)
SPANXC/CT11.3 testis (2.0), placenta (0.10), pancreas (1.2), liver (0.19)
HAGE/CT13 testis (0.02), pancreas (0.01)
ADAM2/CT15 testis (0.03), pancreas (0.02)
SGY-1/CT34 testis (1.87), ovary (0.01), pancreas (0.03), spleen (0.01)
NY-SAR-35/CT37 testis (1.3), placenta (0.01), pancreas (0.02), liver (0.01)
TDRD1/CT41.1 testis (0.08), pancreas (0.02), spleen (0.01)
Differentially Expressedc CT Genes
CT Transcript Tissue (µg cDNA product)
XAGE-1a/CT12.1a testis (>2.0), placenta (0.03), pancreas (0.02), spleen (0.02), thymus (0.02), lung (>2.0), skeletal muscle (0.01)
PAGE-5/CT16.1 testis (0.07), pancreas (0.01), spleen (0.01), kidney (0.02), liver (0.02)
CAGE/CT26 testis (0.23), pancreas (0.03), leukocytes (0.01), brain (0.01)
BORIS/CT27 testis (>2.0), pancreas (0.03), prostate (0.01), thymus (0.01), kidney (0.02)
D40/CT29 testis (0.05), placenta (0.02), pancreas (0.01), thymus (0.02), colon (0.01), lung (0.01)
NXF2/CT39 testis (>2.0), pancreas (0.02), prostate (0.02), brain (0.02), kidney (0.02), lung (0.03)
TEX15/CT42 testis (>2.0), ovary (0.02), pancreas (0.07), spleen (0.02), liver (0.06), small intestine (0.06), prostate (0.01)
FATE/CT43 testis (>2.0), ovary (0.01), thymus (0.06), heart (0.02), kidney (0.02), lung (0.02), skeletal muscle (0.02), pancreas (0.02)
TAF7L/CT40 testis (0.15), placenta (0.03), pancreas (0.03), prostate (0.01), brain (0.01), kidney (0.03), liver (0.03), small intestine (0.02)
Ubiquitously Expressed CT Genes (Figure 1)
IL-13r alpha/CT19, SPA17/CT22, PLU-1/CT31, TSP50/CT20, OY-TES-1/CT23

aCommercially available, normalized cDNA (MTC panels, BD Biosciences, Palo Alto CA) derived from 13 non-gametogenic tissues (colon, leukocyte, prostate, spleen, thymus, brain, heart, kidney, liver, lung, skeletal muscle, pancreas and small intestine), as well as testis, ovary and placenta. b35 cycles of PCR amplification were performed using primer sequences and annealing temperatures described in primary publications. cDifferentially Expressed CT Transcripts were detected in 3-6 of the 13 non-gametogenic tissues analyzed.