Table 3

MHC class I and HLA-A2 expression in tumor cell lines.

Cell line HLA-A2 typing MHC class I expression a HLA-A2 expression b
Isotype control - IFN-gamma + IFN-gamma Isotype control - IFN-gamma + IFN-gamma
SK-ES-1 + 5 20 325 2 6 134
SK-LMS-1 + 7 184 543 6 107 551
SK-UT-1 + 6 263 412 4 5 8
SW 872 + 6 84 121 5 187 630
Saos-2 + 6 118 150 4 84 150
HOS + 11 183 230 7 244 558
MES-SA - 6 28 245 3 5 6
HT-1080 - 5 36 330 4 8 8
SK-MEL-37 + 4 78 158 4 38 63
Mel 275 + 5 76 * c 4 75 * c

aMHC-class-I expression was assessed by staining with mAb W6/32. The mean fluorescence intensity is reported. bHLA-A2 expression was assessed by staining with mAb BB7.2. The mean fluorescence intensity is reported. cCells died upon treatment with IFN-gamma.