Table 3

Primer sequences and PCR conditions used.

Genes Primers PCR Conditions No. of Cycles Product Size (bp)
NY-ESO-1 5'-CAGGGCTGAATGGATGCTGCAGA-3' 5'-GCGCCTCTGCCCTGAGGGAGG-3' 94°C, 1 min 60°C, 1 min 72°C, 1 min 35 332
LAGE-1 5'-CTGCGCAGGATGGAAGGTGCCCC-3' 5'-GCGCCTCTGCCCTGAGGGAGC-3' 94°C, 1 min 60°C, 1 min 72°C, 1 min 35 332
MAGE-1 5'-CGGCCGAAGGAACCTGACCCAG-3' 5'-GCTGGAACCCTCACTGGGTTGCC-3' 94°C, 1 min 72°C, 1 min 72°C, 2 min 35 421
MAGE-3 5'-GAAGCCGGCCCAGGCCCAGGCTCG-3' 5'-GGACGATTATCAGGAGGCCTGC-3' 94°C, 1 min 66°C, 1 min 72°C, 2 min 35 778
MAGE-4 5'-GAGCAGACAGGCCAACCG-3' 5'-AAGGACTCTGCGTCAGGC-3' 94°C, 30 s 68°C, 30 s 72°C, 30 s 35 446
SCP-1/ HOM-TES-14 5'-GTACAGCAGAAAGCAAGCAACTGAATG-3' 5'-GAAGGAACTGCTTTAGAATCCAATTTCC-3' 94°C, 1 min 54°C, 1 min 72°C, 1 min 40 565
SSX-1 5'-CTAAAGCATCAGAGAAGAGAAGC-3' 5'-AGATCTCTTATTAATCTTCTCAGAAA-3' 94°C, 1 min 56°C, 1 min 72°C, 1 min 40 422
SSX-2/ HOM-MEL-40 5'-GTGCTCAAATACCAGAGAAGATC-3' 5'-TTTTGGGTCCAGATCTCTCGTG-3' 94°C, 1 min 67°C, 1 min 72°C, 1 min 35 435
SSX-4 5'-AAATCGTCTATGTGTATATGAAGCT-3 5'-GGGTCGCTGATCTCTTCATAA-3' 94°C, 1 min 60°C, 1 min 72°C, 1 min 35 415
CT7/ MAGE-C1 5'-GACGAGGATCGTCTCAGGTCAGC-3' 5'-ACATCCTCACCCTCAGGAGGG-3' 94°C, 1 min 60°C, 1 min 72°C, 1 min 35 632
CT10/ MAGE-C2/ HCA587 5'-TGTACTGCGCTGCCGTGAGACTGGTG-3' 5'-AAGCTCCATGAACTCACGGGCTCTCTTG-3' 94°C, 1 min 68°C, 1 min 72°C, 1 min 35 752
G3PDH (control) 5'-GTCAACGGATTTGGTCGTATT-3' 5'-AGTCTTCTGGGTGGCAGTGAT-3' 94°C, 1 min 56°C, 1 min 72°C, 1 min 24 540