Table 1

Comparison of different serological technologies for the detection of anti-NY-ESO-1 immune responsesa.

Sample No. RAYSb ELISAc Western Blotd Phage Displayd
FACS Ratio
F 1 49.68 21.6 1284 +++ +
F 2 3.12 1.28 421 - -
F 3 11.63 4.02 410 ++ -
F 4 3.2 1.47 589 - +
F 5 12.88 5.37 1064 ++ +
F 6 57.11 16.22 1008 +++ +
F 7 2.78 1.22 201 - -
F 8 2.97 1.28 260 - -
F 9 2.89 1.23 316 - -
F 10 9.49 4.12 884 + +
F 11 6.12 2.18 742 + +
F 12 11.45 3.78 1003 ++ +
F 13 35.38 15.72 1537 ++ +
F 14 3.3 2.64 658 + -
F 15 5.57 2.71 808 + -
F 16 5.13 2.26 534 + +
F 17 28.84 13.54 1389 +++ +
F 18 8.03 2.84 812 ++ +
F 19 6.65 3.69 1161 + +
F 20 62.04 27.45 1037 +++ +
F 21 63.75 27.48 1014 +++ +
F 22 4.22 2.11 449 + +
F 23 18.95 7.52 1122 +++ +
F 24 47.55 19.02 997 +++ +
F 25 13.67 5.69 1595 +++ +
F 26 31.23 13.17 1299 ++ +
F 27 59.12 26.6 1340 +++ +
F 28 2.72 2.16 772 + -
F 29 9.1 3.8 874 + -
F 30 6.49 2.58 274 (+) -
F 31 3 1.15 246 - -
F 32 2.85 1.84 302 - -
F 33 3 1.26 215 - -
F 34 3 1.16 137 - -
F 35 3.99 1.54 280 - -
F 36 4.5 1.48 429 - -
F 37 3.2 1.22 593 - -
F 38 3.4 1.27 271 - -
F 39 3.3 1.34 274 - -
F 40 3.7 1.35 225 - -
F 41 3 1.18 133 - -
F 42 <0 1.52 312 - -
F 43 4.1 1.45 194 - -
F 44 3.1 0.8 453 - -
F 45 3.4 1.31 224 - -
F 46 3.4 1.29 346 - -
F 47 3.4 1.27 352 - -
F 48 2.2 1.6 360 - -
F 49 3.1 1.18 287 - -
F 50 3.5 0.34 199 - -

aFifty serum samples from patients with different malignancies were analyzed in a blind fashion. Serum samples were diluted 1:100 for RAYS and phage display, 1:400 for ELISA and 1:250 for Western blot analysis. Individual values highlighted in bold are considered positive. bAntibody binding to yeast was determined by FACS and the mean fluorescence intensity values are given. The RAYS ratio was determined by dividing the fluorescence intensity obtained by the individual serum sample on NY-ESO-1 protein positive yeast by the fluorescence intensity that was obtained on yeast expressing an irrelevant antigen. Samples with a ratio of two or more were considered positive. cThe mean ELISA values (OD at 405 nm) of triplicate analyses are given. dThe intensity of the signal detected by Western blotting or phage analysis was determined visually.