Table 2

Comparison of different serological technologies for the detection of anti-SSX-2 immune responsesa.

Sample No. RAYS ELISA
FACS Ratio
1 46.9 20.9 486
2 2 0.75 191
3 3 0.72 118
4 2 0.82 239
5 15.1 6.5 187
6 80.1 30.23 230
7 3 0.87 154
8 2 0.83 230
9 4 0.74 134
10 29.5 10.5 189

aSerum samples from 10 patients with solid cancers were analyzed for the presence of anti-SSX-2 antibodies by ELISA (1:400 dilution) and RAYS (1:100 dilution) as described. Positive samples are highlighted in bold. The intensity of the signal obtained by the individual assay and the yeast ratio were determined as described for Table 1. The ELISA data shown are mean values of triplicate analyses.