Resistance of Meth A tumor cells to lysis by H-2d-specific CTLs1
| Target Cells | % 51Cr release at E:T ratios: | |||||
| Exp. 1 | Exp. 2 | |||||
| 20 | 10 | 5 | 20 | 10 | 5 | |
| BALB/cJ Con A blasts2 | 68 | 65 | 52 | 69 | 55 | 27 |
| C57BL/6 Con A blasts2 | 0 | 0 | 1 | 4 | 0 | 0 |
| C3H-HeN Con A blasts2 | 12 | 10 | 0 | 20 | 14 | 11 |
| 10ME | 73 | 62 | 49 | 72 | 51 | 29 |
| Meth A ascites | 12 | 8 | 0 | 0 | 0 | 3 |
| Meth A cultured | 25 | 19 | 9 | 17 | 10 | 7 |
| CMS4 | 60 | 49 | 37 | - | - | - |
| CMS5 | 46 | 34 | 23 | 50 | 34 | 20 |
1C57BL/6 mice were challenged intradermally with 2 x 106 live Meth A cells. After tumor rejection, spleen cells were harvested and cultured with mitomycin C-treated Meth A cells (responder to stimulator ratio 20:1). On day 6 CTLs were tested against the indicated targets in 51Cr release assays. Shown are two representative experiments, of a total of five. 2Con A blasts was prepared by culturing 2 x 107 whole spleen cells in 10 ml complete RPMI with Concanavalin A (final concentration 5 µg/ml). Blasts were harvested after 48 hours and 51Cr-labeled for use as target cells in CTL assays.