Table 1.

Interassay coefficients of variation in quantitative RT-PCR assays for MIR21 and RNU6-2

Targets in quantitative RT-PCR assays
MIR21RNU6-2
Mean cycle threshold ± SDInterassay coefficient of variation (%)Mean cycle threshold ± SDInterassay coefficient of variation (%)
Specimen 119.5 ± 0.060.2819.9 ± 0.130.65
Specimen 219.5 ± 0.070.3621.2 ± 0.150.73
Specimen 319.3 ± 0.090.4421.0 ± 0.180.86
Specimen 420.0 ± 0.090.4622.1 ± 0.090.39
Specimen 518.0 ± 0.100.5521.0 ± 0.120.59
Mean coefficient of variation (%)0.420.64

NOTE: Interassay coefficient of variation of cycle threshold values from the same specimen were assessed by repeating assays in five different batches with the use of five colorectal cancers.