Distribution of colorectal cancer cases according to tumor MIR21 expression and the density of T cells
| Tumor MIR21 expression (quartile) | ||||||
|---|---|---|---|---|---|---|
| Total | Q1 (lowest) | Q2 (second) | Q3 (third) | Q4 (highest) | Ptrenda | |
| CD3+ cell density (quartile) | 0.0004 | |||||
| Q1 (0–115 cells/mm2) | 130 (25%) | 26 (20%) | 29 (22%) | 31 (25%) | 44 (33%) | |
| Q2 (116–252 cells/mm2) | 129 (25%) | 26 (20%) | 30 (23%) | 35 (28%) | 38 (29%) | |
| Q3 (253–533 cells/mm2) | 130 (25%) | 42 (32%) | 33 (25%) | 30 (24%) | 25 (19%) | |
| Q4 (≥534 cells/mm2) | 129 (25%) | 38 (28%) | 38 (30%) | 28 (23%) | 25 (19%) | |
| CD8+ cell density (quartile) | 0.27 | |||||
| Q1 (0–66 cells/mm2) | 128 (25%) | 23 (18%) | 31 (24%) | 40 (32%) | 34 (26%) | |
| Q2 (67–185 cells/mm2) | 127 (25%) | 42 (33%) | 26 (20%) | 28 (23%) | 31 (24%) | |
| Q3 (186–410 cells/mm2) | 128 (25%) | 30 (24%) | 34 (26%) | 28 (23%) | 36 (27%) | |
| Q4 (≥411 cells/mm2) | 127 (25%) | 31 (25%) | 39 (30%) | 27 (22%) | 30 (23%) | |
| CD45RO+ cell density (quartile) | 0.0002 | |||||
| Q1 (0–183 cells/mm2) | 131 (25%) | 24 (18%) | 30 (23%) | 31 (24%) | 46 (35%) | |
| Q2 (184–430 cells/mm2) | 130 (25%) | 32 (25%) | 33 (25%) | 39 (31%) | 26 (20%) | |
| Q3 (431–805 cells/mm2) | 131 (25%) | 26 (20%) | 37 (27%) | 30 (24%) | 38 (29%) | |
| Q4 (≥806 cells/mm2) | 130 (25%) | 48 (37%) | 33 (25%) | 27 (21%) | 22 (16%) | |
| FOXP3+ cell density (quartile) | 0.032 | |||||
| Q1 (0–14 cells/mm2) | 124 (25%) | 25 (20%) | 31 (26%) | 32 (27%) | 36 (28%) | |
| Q2 (15–25 cells/mm2) | 124 (25%) | 29 (23%) | 24 (20%) | 34 (28%) | 37 (28%) | |
| Q3 (26–48 cells/mm2) | 124 (25%) | 38 (31%) | 27 (22%) | 27 (22%) | 32 (25%) | |
| Q4 (≥49 cells/mm2) | 123 (25%) | 32 (26%) | 38 (32%) | 28 (23%) | 25 (19%) | |
Abbreviations: Q1 to Q4, quartile 1 to quartile 4.
↵aPtrend value was calculated by the linear trend test across the ordinal (first to fourth quartile) categories of tumor MIR21 expression as a continuous variable in a univariable ordinal logistic regression model for the density of CD3+, CD8+, CD45RO+, or FOXP3+ T cells (an ordinal quartile outcome variable). Because we assessed four primary outcome variables, we adjusted the two-sided α level to 0.012 (= 0.05/4) by simple Bonferroni correction.