Table 3.

Distribution of colorectal cancer cases according to tumor MIR21 expression and the density of T cells

Tumor MIR21 expression (quartile)
TotalQ1 (lowest)Q2 (second)Q3 (third)Q4 (highest)Ptrenda
CD3+ cell density (quartile)0.0004
 Q1 (0–115 cells/mm2)130 (25%)26 (20%)29 (22%)31 (25%)44 (33%)
 Q2 (116–252 cells/mm2)129 (25%)26 (20%)30 (23%)35 (28%)38 (29%)
 Q3 (253–533 cells/mm2)130 (25%)42 (32%)33 (25%)30 (24%)25 (19%)
 Q4 (≥534 cells/mm2)129 (25%)38 (28%)38 (30%)28 (23%)25 (19%)
CD8+ cell density (quartile)0.27
 Q1 (0–66 cells/mm2)128 (25%)23 (18%)31 (24%)40 (32%)34 (26%)
 Q2 (67–185 cells/mm2)127 (25%)42 (33%)26 (20%)28 (23%)31 (24%)
 Q3 (186–410 cells/mm2)128 (25%)30 (24%)34 (26%)28 (23%)36 (27%)
 Q4 (≥411 cells/mm2)127 (25%)31 (25%)39 (30%)27 (22%)30 (23%)
CD45RO+ cell density (quartile)0.0002
 Q1 (0–183 cells/mm2)131 (25%)24 (18%)30 (23%)31 (24%)46 (35%)
 Q2 (184–430 cells/mm2)130 (25%)32 (25%)33 (25%)39 (31%)26 (20%)
 Q3 (431–805 cells/mm2)131 (25%)26 (20%)37 (27%)30 (24%)38 (29%)
 Q4 (≥806 cells/mm2)130 (25%)48 (37%)33 (25%)27 (21%)22 (16%)
FOXP3+ cell density (quartile)0.032
 Q1 (0–14 cells/mm2)124 (25%)25 (20%)31 (26%)32 (27%)36 (28%)
 Q2 (15–25 cells/mm2)124 (25%)29 (23%)24 (20%)34 (28%)37 (28%)
 Q3 (26–48 cells/mm2)124 (25%)38 (31%)27 (22%)27 (22%)32 (25%)
 Q4 (≥49 cells/mm2)123 (25%)32 (26%)38 (32%)28 (23%)25 (19%)

Abbreviations: Q1 to Q4, quartile 1 to quartile 4.

  • aPtrend value was calculated by the linear trend test across the ordinal (first to fourth quartile) categories of tumor MIR21 expression as a continuous variable in a univariable ordinal logistic regression model for the density of CD3+, CD8+, CD45RO+, or FOXP3+ T cells (an ordinal quartile outcome variable). Because we assessed four primary outcome variables, we adjusted the two-sided α level to 0.012 (= 0.05/4) by simple Bonferroni correction.