Table 1.

Summary of antibodies and conditions of use

AntibodyCloneDilutionSourcePretreatment
ER6F111/75VectorTris buffer (pH 9.0)
PgRPgR 12941/1,000DAKOTris buffer (pH 9.0)
p53D.071/400ID LabTris buffer (pH 9.0)
CK5XM261/400VectorTris buffer (pH 9.0)
HER2CB11 TAB250 (cocktail)1/300NovocastraPepsin 10 minutes at 37°C
1/300Zymed
EGFR31G71/25ZymedPepsin 10 minutes at 37°C
Ki67MIB11/300DAKOCitrate buffer (pH 6.0)
T-bet4B101/100Santa Cruz BiotechnologyCitrate buffer (pH 6.0)