Table 1.

Biochemical characterization of selected tumor antigens detected by postinfection antibodies

Spot number	Accession number^a	Protein name^b	Peptide sequence^c	X-corr^d	Δ Score^e	Charge^f	M/Z (Da)^g	MH+^h (Da)	ΔMⁱ (ppm)	MW^j (kDa)	pI^k	% Sequence coverage^l	Ions matched^m
2	IPI00885367.1	Annexin A2	TNQELQEINR	2.74	0.36	2	622.81384	1244.62041	−2.08	19.6	5.96	10.80	14/18
			QDIAFAYQR	2.55	1.00	2	556.28143	1111.55559	2.22				14/16
3	IPI00468203.3	Annexin A2	TPAQYDASELK	3.52	0.65	2	611.80090	1222.59453	−0.43	38.7	7.69	22.71	15/20
			AKDGSVIDYELIDQDAR	3.46	0.68	2	954.94586	1908.88445	1.16				17/32
			SLYYYIQQDTK	3.30	0.47	2	711.35215	1421.69704	1.62				16/20
			TNQELQEINR	3.08	0.47	2	622.81384	1244.62041	−2.08				15/18
			WISIMTER	2.67	1.00	2	518.26892	1035.53057	1.29				13/14
			DIISDTSGDFR	2.53	1.00	2	613.28820	1225.56914	−0.36				16/20
			QDIAFAYQR	2.41	0.60	2	556.28027	1111.55327	0.13				15/16
4	IPI00407339.7	Histone H4	ISGLIYEETR	3.39	0.68	2	590.81536	1180.62346	2.19	11.4	11.36	38.83	16/18
			TVTAMDWYALK	3.22	0.74	2	655.85559	1310.70391	1.06				16/22
			TVTAmDVVYALK	2.88	0.42	2	663.85327	1326.69927	1.39				14/22
			DAVTYTEHAK	2.61	0.52	2	567.77410	1134.54094	−1.47				15/18
			VFLENVIR	2.56	0.36	2	495.29345	989.57964	1.76				12/14
4	IPI00622795.2	GAPDH	LENPAKYDDIK	2.36	8.03	2	653.33881	1305.67035	0.20	35.8	8.03	3.30	10/20
4	IPI00323592.2	MDH2	IFGVTTLDIVR	2.82	0.41	2	617.36487	1233.72246	0.41	35.6	8.68	3.25	14/20

5	IPI00885367.1	Annexin A2	TNQELQEINR	3.06	0.47	2	622.81384	1244.62041	−2.08	19.6	5.96	5.68	14/18

↵^aProtein Accessions in the IPI mouse database.
↵^bProtein name.
↵^cPrimary amino acid sequence for identified peptides.
↵^dThe cross-correlation of the experimental and theoretical spectra from SEQUEST search.
↵^eA measure of the difference between the top two scores for the peptides identified by that spectrum.
↵^fCharge stage of precursor ion.
↵^gObserved mass-to-charge ratio of precursor ion.
↵^hMonoisotopic mass for protonated molecular ion MH calculated on the basis of observed m/z.
↵ⁱDeviation from expected MH.
↵^jMolecular weight.
↵^kIsoelectric point.
↵^lPercentage of the database protein sequence covered by matching peptides.
↵^mThe number of fragment ions matched out of the total number of fragment masses for the peptide.