Table 1.

Biochemical characterization of selected tumor antigens detected by postinfection antibodies

Spot numberAccession numberaProtein namebPeptide sequencecX-corrdΔ ScoreeChargefM/Z (Da)gMH+h (Da)ΔMi (ppm)MWj (kDa)pIk% Sequence coveragelIons matchedm
2IPI00885367.1Annexin A2TNQELQEINR2.740.362622.813841244.62041−2.0819.65.9610.8014/18
QDIAFAYQR2.551.002556.281431111.555592.2214/16
3IPI00468203.3Annexin A2TPAQYDASELK3.520.652611.800901222.59453−0.4338.77.6922.7115/20
AKDGSVIDYELIDQDAR3.460.682954.945861908.884451.1617/32
SLYYYIQQDTK3.300.472711.352151421.697041.6216/20
TNQELQEINR3.080.472622.813841244.62041−2.0815/18
WISIMTER2.671.002518.268921035.530571.2913/14
DIISDTSGDFR2.531.002613.288201225.56914−0.3616/20
QDIAFAYQR2.410.602556.280271111.553270.1315/16
4IPI00407339.7Histone H4ISGLIYEETR3.390.682590.815361180.623462.1911.411.3638.8316/18
TVTAMDWYALK3.220.742655.855591310.703911.0616/22
TVTAmDVVYALK2.880.422663.853271326.699271.3914/22
DAVTYTEHAK2.610.522567.774101134.54094−1.4715/18
VFLENVIR2.560.362495.29345989.579641.7612/14
4IPI00622795.2GAPDHLENPAKYDDIK2.368.032653.338811305.670350.2035.88.033.3010/20
4IPI00323592.2MDH2IFGVTTLDIVR2.820.412617.364871233.722460.4135.68.683.2514/20
5IPI00885367.1Annexin A2TNQELQEINR3.060.472622.813841244.62041−2.0819.65.965.6814/18
  • aProtein Accessions in the IPI mouse database.

  • bProtein name.

  • cPrimary amino acid sequence for identified peptides.

  • dThe cross-correlation of the experimental and theoretical spectra from SEQUEST search.

  • eA measure of the difference between the top two scores for the peptides identified by that spectrum.

  • fCharge stage of precursor ion.

  • gObserved mass-to-charge ratio of precursor ion.

  • hMonoisotopic mass for protonated molecular ion MH calculated on the basis of observed m/z.

  • iDeviation from expected MH.

  • jMolecular weight.

  • kIsoelectric point.

  • lPercentage of the database protein sequence covered by matching peptides.

  • mThe number of fragment ions matched out of the total number of fragment masses for the peptide.