RT Journal Article SR Electronic T1 Infiltration by IL22-Producing T Cells Promotes Neutrophil Recruitment and Predicts Favorable Clinical Outcome in Human Colorectal Cancer JF Cancer Immunology Research JO Cancer Immunol Res FD American Association for Cancer Research SP 1452 OP 1462 DO 10.1158/2326-6066.CIR-19-0934 VO 8 IS 11 A1 Tosti, Nadia A1 Cremonesi, Eleonora A1 Governa, Valeria A1 Basso, Camilla A1 Kancherla, Venkatesh A1 Coto-Llerena, Mairene A1 Amicarella, Francesca A1 Weixler, Benjamin A1 Däster, Silvio A1 Sconocchia, Giuseppe A1 Majno, Pietro Edoardo A1 Christoforidis, Dimitri A1 Tornillo, Luigi A1 Terracciano, Luigi A1 Ng, Charlotte K.Y. A1 Piscuoglio, Salvatore A1 von Flüe, Markus A1 Spagnoli, Giulio A1 Eppenberger-Castori, Serenella A1 Iezzi, Giandomenica A1 Droeser, Raoul Andre YR 2020 UL http://cancerimmunolres.aacrjournals.org/content/8/11/1452.abstract AB Immune cell infiltration in colorectal cancer effectively predicts clinical outcome. IL22, produced by immune cells, plays an important role in inflammatory bowel disease, but its relevance in colorectal cancer remains unclear. Here, we addressed the prognostic significance of IL22+ cell infiltration in colorectal cancer and its effects on the composition of tumor microenvironment. Tissue microarrays (TMA) were stained with an IL22-specific mAb, and positive immune cells were counted by expert pathologists. Results were correlated with clinicopathologic data and overall survival (OS). Phenotypes of IL22-producing cells were assessed by flow cytometry on cell suspensions from digested specimens. Chemokine production was evaluated in vitro upon colorectal cancer cell exposure to IL22, and culture supernatants were used to assess neutrophil migration in vitro. Evaluation of a testing (n = 425) and a validation TMA (n = 89) revealed that high numbers of IL22 tumor-infiltrating immune cells were associated with improved OS in colorectal cancer. Ex vivo analysis indicated that IL22 was produced by CD4+ and CD8+ polyfunctional T cells, which also produced IL17 and IFNγ. Exposure of colorectal cancer cells to IL22 promoted the release of the neutrophil-recruiting chemokines CXCL1, CXCL2, and CXCL3 and enhanced neutrophil migration in vitro. Combined survival analysis revealed that the favorable prognostic significance of IL22 in colorectal cancer relied on the presence of neutrophils and was enhanced by T-cell infiltration. Altogether, colorectal cancer–infiltrating IL22-producing T cells promoted a favorable clinical outcome by recruiting beneficial neutrophils capable of enhancing T-cell responses.