PT  - JOURNAL ARTICLE
AU  - Wang, Ting
AU  - Wang, Changxi
AU  - Wu, Jinghua
AU  - He, Chenyang
AU  - Zhang, Wei
AU  - Liu, Jiayun
AU  - Zhang, Ruifang
AU  - Lv, Yonggang
AU  - Li, Yongping
AU  - Zeng, Xiaojing
AU  - Cao, Hongzhi
AU  - Zhang, Xiuqing
AU  - Xu, Xun
AU  - Huang, Chen
AU  - Wang, Ling
AU  - Liu, Xiao
TI  - The Different T-cell Receptor Repertoires in Breast Cancer Tumors, Draining Lymph Nodes, and Adjacent Tissues
AID  - 10.1158/2326-6066.CIR-16-0107
DP  - 2017 Feb 01
TA  - Cancer Immunology Research
PG  - 148--156
VI  - 5
IP  - 2
4099  - http://cancerimmunolres.aacrjournals.org/content/5/2/148.short
4100  - http://cancerimmunolres.aacrjournals.org/content/5/2/148.full
SO  - Cancer Immunol Res2017 Feb 01; 5
AB  - T lymphocytes infiltrate the microenvironment of breast cancer tumors and play a pivotal role in tumor immune surveillance. Relationships between the T-cell receptors (TCR) borne by T cells within tumors, in the surrounding tissues, and in draining lymph nodes are largely unexplored in human breast cancer. Consequently, information about the relative extent of possible T-cell exchange between these tissues is also lacking. Here, we have analyzed the TCR repertoire of T cells using multiplex PCR and high-throughput sequencing of the TCRβ chain in the tissues of tumor, adjacent nontumor, and axillary lymph nodes of breast cancer patients. T-cell repertoire diversity in tumors was lower than in lymph nodes, but higher than in nontumor tissue, with a preferential use of variable and joining genes. These data are consistent with the hypothesis that most of the T cells in tumors derive from the lymph node, followed by their expansion in tumor tissue. Positive nodes appeared to enhance T-cell infiltration into tumors and T-cell clonal expansion in lymph nodes. Additionally, the similarity in TCR repertoire between tumor and nontumor tissue was significantly higher in luminal-like, rather than basal-like, breast cancer. Our study elucidated the high heterogeneity of the TCR repertoire and provides potential for future improvements in immune-related diagnosis, therapy, and prognosis for breast cancer patients. Cancer Immunol Res; 5(2); 148–56. ©2016 AACR.