Article Figures & Data
Figures
- Figure 1
RT-PCR analysis for PLAC1 and DPPA2 expression in a normal tissue panel. (A) PLAC1 mRNA expression yielded a 328 bp product. (B) DPPA2 mRNA expression yielded a 430 bp product. (C) GAPDH (glyceraldehyde-3-phosphate dehydrogenase) served as an endogenous control and yielded a 204 bp product. Lanes: M, marker; 1, blank; 2, placenta; 3, adrenal gland; 4, bone marrow; 5, brain; 6, cerebellum; 7, fetal liver; 8, fetal brain; 9, heart; 10, placenta; 11, prostate; 12, testis.
- Table 1
Patient characteristics.
- Figure 2
RT-PCR analysis for PLAC1 expression in a subset of ovarian cancer specimens.PLAC1 mRNA expression yielded a 328 bp product. GAPDH (glyceraldehyde-3-phosphate dehydrogenase) served as an endogenous control and yielded a 204 bp product. Shown is mRNA from placenta (lane 1). Lanes 2, 4, 6, and 7 are positive for PLAC1 mRNA expression whereas lanes 3, 8, and 9 are negative. Lane 5 has a negative GAPDH control and hence PLAC1 mRNA expression in this specimen is inconclusive.
- Figure 3
DPPA2 expression in testis and an ovarian tumor specimen. Immunohistochemical staining was carried out with a polyclonal rabbit anti-DPPA2 antibody. (A) The spermatocytes of the normal testis show a nuclear pattern of staining of strong intensity. (B) Staining of an ovarian tumor specimen.
- Figure 4
Serological reactivity of the recombinant PLAC1 protein in a subset of ovarian cancer patients. The presence of anti-PLAC1 antibodies in serum samples was assessed by ELISA. Serum obtained from a healthy donor (#00100212) was used as a known positive control.
- Figure 5
Serological reactivity of the recombinant DPPA2 protein in a subset of ovarian cancer patients. The presence of anti-DPPA2 antibodies in serum samples was assessed by ELISA. Serum obtained from a seropositive NY-ESO-1 patient (SRM13) was used as a positive control and that obtained from a seronegative NY-ESO-1 patient (SRM24) was used as a negative control. Sera #15559, #15555, and #15418 tested negative for DPPA2 expression by RT-PCR.
Volume 9, Issue 1
