Article Figures & Data
Figures
- Figure 1
Induction of NY-ESO-1-specific CD4+ and CD8+ T cell responses after in vitro presensitization with NY-ESO-1 p157-170 or Ad2/ESO. CD4+ and CD8+ T cells were presensitized with (a) NY-ESO-1 p157-170 or (b) Ad2/ESO. Two patients, NW1454 and NW1691, showed nonspecific reactivity against the irrelevant Melan A peptide p29-37 (white bars) after Ad2/ESO stimulation (a and b). (c) CD8+ T cell responses were induced after presensitization with NY-ESO-1 p157-170 against the stimulating peptide (black bar), and against T2 cells pulsed with NY-ESO-1 p157-165 (checkered bars) and p157-167 (vertical stripes). No significant reactivity was observed against the irrelevant Melan A peptide p29-37 (white bars) or against T2 cells alone (horizontal stripes).
- Figure 2
NY-ESO-1 p157-170-specific CD4+ T cell reactivity is restricted by HLA-DP4. The CD4+ T cell clone NW 1662-CD4-12 was tested against a panel of HLA-DP4-positive and HLA-DP4-negative allogeneic EBV-B cell lines pulsed with NY-ESO-1 p157-170 (black bars) in ELISPOT assays. NY-ESO-1 p157-170-specific reactivity was documented against peptide-pulsed HLA-DP4+ EBV-B cells only. The white bars represent the background reactivity against EBV-B cells alone.
- Figure 3
Reactivity of HLA-DP4-restricted NY-ESO-1-specific CD4+ T cells clones against different concentrations of NY-ESO-1 p157-170. Seven CD4+ T cell clones obtained from patients NW1454 and NW1662 were tested against different concentrations of NY-ESO-1 p157-170 in ELISPOT assays. Depending upon the interclonal avidity, different levels of peptide concentration are required for detectable reactivity with the respective CD4+ T cell clone.
- Figure 4
Recognition of naturally processed NY-ESO-1. (a) A panel of CD4+ T cell clones were tested in ELISPOT assays against autologous DCs pulsed with recombinant NY-ESO-1 protein (black bars) or irrelevant recombinant SSX protein (white bars), or infected with Ad2/ESO (horizontal stripes), v.v. ESO (checkered bars), or v.v. WT (gray bars). (b) CD4+ T cell clone NW 1662-CD4-2 was tested for specific recognition of autologous DCs pulsed with lysates prepared from NY-ESO-1-positive (NW-MEL-38 and SK-MEL-37) or NY-ESO-1-negative (NW-MEL-8) melanoma cell lines in ELISPOT assays.
- Figure 5
NY-ESO-1-specific cytotoxic reactivity of CD4+ T cell clones. (a) The CD4+ T cell clones NW 1454-CD4-68 (triangles) and NW 1662-CD4-2 (squares) were tested against the allogenic HLA-DP4+ EBV-B cell line NW1539-EBV-B, either pulsed with NY-ESO-1 p157-170 or not (closed and open symbols, respectively) in a 4-h chromium release assay at E/T ratios of 90, 30, 10, and 1:1. (b) The CD4+ T cell clone NW 1454-CD4-68 was tested against the HLA-DP4+ NY-ESO-1-positive melanoma cell lines NW-MEL-38, NW-MEL 634, and NW-MEL-450, against the NY-ESO-1-negative melanoma cell line NW-MEL-8, and against the HLA-DP4-negative melanoma cell line SK-MEL-29 in a 4-h chromium release assay.
Tables
- Table 1
NY-ESO-1 antibody and HLA-DP4 status of patients with NY-ESO-1-expressing cancers.
HLA-DP4 status NY-ESO-1 Ab status Ab+ patients (n=50) Ab- patients (n=52) HLA-DP4+ 39 40 HLA-DP4- 11 12
Volume 4, Issue 1
