Article Figures & Data
Figures
- Figure 1
Expression profile of the ubiquitously expressed CT genes IL13RA/CT19, TSP50/CT20, OY-TES-1/CT23, and PLU-1/CT31. A standardized, quantitative end-point RT-PCR assay was used to evaluate the mRNA expression profile of IL13RA/CT19, TSP50/CT20, OY-TES-1/CT23, and PLU-1/CT31 in a series of 16 normal tissues (MTC panels I and II, BD Biosciences, Palo Alto CA). The level of PLU-1/CT31 expression was low in all tissues with the exception of testis. TSP50/CT20 transcripts were detected at high levels in testis, kidney, liver, and pancreas; at a moderate level in thymus and at low levels in all other normal tissues. Transcripts encoding IL13RA/CT19, SPA17/CT22, and OY-TES-1/CT23 were detected at high levels in 11/13 non-gametogenic tissues.
- Figure 2
Verification of CT expression in normal pancreas by RT-PCR. End-point RT-PCR assay was used to verify the mRNA expression of 25 CT transcripts in a second sample of pancreatic cDNA (see text). The presence of mRNA encoding MAGEA1/CT1.1, XAGE-1a/CT12.1a, HAGE/CT13, PAGE-5/CT16A, IL13RA/CT19, TSP50/CT20, SPA17/CT22, OY-TES-1/CT23, BORIS/CT27, D40/CT29, PLU-1/CT31, SGY-1/CT34, NY-SAR-35/CT37, NXF2/CT39, TAF7L/CT40, and TDRD1/CT41.1 was confirmed in normal pancreas. Lane assignments are as follows: 1, OY-TES-1/CT23; 2, MAGEC1/CT7.1; 3, NY-ESO-1/CT6.1; 4, SYCP1/CT8; 5, BORIS/CT27; 6, ADAM2/CT15; 7, IL13RA/CT19; 8, PLU-1/CT31; 9, TDRD1/CT41.1; 10, TEX15/CT42; 11, MAGEA1/CT1.1; 12, TSP50/CT20; 13, NXF2/CT39; 14, SGY-1/CT34; 15, CAGE/CT26; 16, TAF7L/CT42; 17, D40/CT29; 18, PAGE-5/CT16.1; 19, NY-SAR-35/CT37; 20, SPA17/CT22; 21, HAGE/CT13; 22, SAGE/CT14; 23, SPANXC/CT11.3; 24, XAGE-1a/CT12.1a; 25, FATE/CT43.
Tables
- Table 1
Current list of CT genes: 44 CT gene families, 89 individual genes or isoforms.
Transcript/ Transcript family CT Identifier Family Members/CT Identifier MAGEA CT1 MAGEA1/CT1.1, MAGEA2/CT1.2, MAGEA3/CT1.3, MAGEA4/CT1.4, MAGEA5/CT1.5, MAGEA6/CT1.6, MAGEA7/CT1.7, MAGEA8/CT1.8, MAGEA9/CT.9, MAGEA10/CT1.10, MAGEA11/CT1.11, MAGEA12/CT1.12 BAGE CT2 BAGE/CT2.1, BAGE2/CT2.2, BAGE3/CT2.3, BAGE4/CT2.4, BAGE5/CT2.5 MAGEB CT3 MAGEB1/CT3.1, MAGEB2/CT3.2, MAGEB5/CT3.3, MAGEB6/CT3.4 GAGE1 CT4 GAGE1/CT4.1, GAGE2/CT4.2, GAGE3/CT4.3, GAGE4/CT4.4, GAGE5/CT4.5, GAGE6/CT4.6, GAGE7/CT4.7, GAGE8/CT4.8 SSX CT5 SSX1/CT5.1, SSX2/CT5.2a, SSX2/CT5.2b, SSX3/CT5.3, SSX4/CT5.4 NY-ESO-1 CT6 NY-ESO-1/CT6.1, LAGE-1a/CT6.2a, LAGE-1b/CT6.2b MAGEC1 CT7 MAGEC1/CT7.1, MAGEC3/CT7.2 SYCP1 CT8 SYCP1/CT8 BRDT CT9 BRDT/CT9 MAGEE1 CT10 MAGEE1/CT10 CTp11/SPANX CT11 SPANXA1/CT11.1, SPANXB1/CT11.2, SPANXC/CT11.3, SPANXD/CT11.4 XAGE-1/GAGED CT12 XAGE-1a/CT12.1a, XAGE-1b/CT12.1b, XAGE-1c/CT12.1c, XAGE-1d/CT12.1d, XAGE-2/CT12.2, XAGE-3a/CT12.3a, XAGE-3b/CT12.3b, XAGE-4/CT12.4 HAGE CT13 HAGE/CT13 SAGE CT14 SAGE/CT14 ADAM2 CT15 ADAM2/CT15 PAGE-5 CT16 PAGE-5/CT16.1, CT16.2 LIP1 CT17 LIP1/CT17 NA88 CT18 NA88/CT12 IL13RA1 CT19 IL13RA1/CT19 TSP50 CT20 TSP50/CT20 CTAGE-1 CT21 CTAGE-1/CT21.1, CTAGE-2/CT21.2 SPA17 CT22 SPA17/CT22 OY-TES-1 CT23 OY-TES-1/CT23 CSAGE CT24 CSAGE/CT24.1, TRAG3/CT24.2 MMA1/DSCR8 CT25 MMA-1a/CT25.1a, MMA-1b/CT25.1b CAGE CT26 CAGE/CT26 BORIS CT27 BORIS/CT27 HOM-TES-85 CT28 HOM-TES-85/CT28 AF15q14/ D40 CT29 D40/CT29 E2F-like/HCA661 CT30 HCA661/CT30 PLU-1 CT31 PLU-1/CT31 LDHC CT32 LDHC/CT32 MORC CT33 MORC/CT33 SGY-1 CT34 SGY-1/CT34 SPO11 CT35 SPO11/CT35 TPX1 CT36 TPX-1/CT36 NY-SAR-35 CT37 NY-SAR-35/CT37 FTHL17 CT38 FTHL17/CT38 NXF2 CT39 NXF2/CT39 TAF7L CT40 TAF7L/CT40 TDRD1 CT41 TDRD1/CT41.1, NY-CO-45/CT41.2 TEX15 CT42 TEX15/CT42 FATE CT43 FATE/CT43 TPTE CT44 TPTE/CT44 - Table 2
CT gene families encoding immunogenic gene products (CT antigens).
Antigen Immune Response MAGEA/CT1 cellular and humoral BAGE/CT2 cellular MAGEB/CT3 humoral GAGE/CT4 cellular SSX/CT5 cellular and humoral NY-ESO-1/CT6 cellular and humoral MAGEC/CT7 humoral SYCP1/CT8 humoral SPANXB1/CT11.2 humoral NA88/CT18 cellular CTAGE/CT21 humoral SPA17/CT22 humoral OY-TES-1/CT23 humoral CAGE/CT26 humoral HOM-TES-85/CT28 humoral HCA661/CT30 humoral NY-SAR-35/CT37 humoral FATE/CT43 humoral TPTE/CT44 humoral - Table 3
Published mRNA expression frequencies of CT genes in various cancersa.
CT Family (Member) Frequency (%) of Expression in Tumor Type Ref Blad Brn Brst Col Eso Gas H/N Liver Leuk/ Lymph Lung (NSCLC) Mel Ov Pancr Pros Renal Sarc MAGEA1/CT1.1 22 - 18 2 53 29 28 80 0 49 48 28 - 15 0 14 44 BAGE1/CT2.1 15 - 10 0 - - 8 - 0 4 26 15 - 0 0 6 44 MAGEB1/CT3.1 0 0 17 0 - 0 0 - 0 14 22 - - 0 0 9 45 GAGE/CT4.1 12 - 9 0 - - 19 38b 1 19 28 31 - 10 0 25 44 SSX2/CT5.2 44 6 7 12 - - 35 9b 36 16 35 - - 40 5 50 46 NY-ESO-1/CT6.1 80 0 30 0 - 0 - 29 0 17 34 25 0 25 9 0 8 MAGEC1/CT7.1 44 - 30 10 - - 36 - - 33 70 - - - - 60 20 SYCP1/CT8 - 47 20 0 - 7 - 28b 0 7 14 0 - 0 8 0 9 BRDT/CT9 0 - 0 0 8 - 8 - - 25 0 - - - 0 - 16 MAGEE1/CT10 44 - 38 0 - - 36 - - 24 50 - - - - 0 12 SPANXC/CT11.3 9 - 25 22 0 - - - - 33 70 - 0 - - - 14 XAGE-1a/CT12.1a - - - - - - - - - - 8 - - - - 22 47 HAGE/CT13 24 37 5 31 27 - - 20 9 32 17 - - 22 6 20 13 SAGE/CT14 12 0 5 0 20 - 17 - 4 22 4 - - 0 5 5 13 ADAM2/CT15 - - 0 0 - - - - - 0 0 0 - - 12 - 17 PAGE-5/CT16 - - 5 11 - - - - - 39 22 0 - - 44 - 17 LIP1/CT17 - - 5 0 - - - - - 0 0 0 - - 25 - 17 NA88/CT18 - - - - - - - - - - 11 - - - - - 48 TSP50/CT20 - - 28 - - - - - - - - - - - - - 49 CTAGE-1/CT21.1 - - - - - - - - 35 - - - - - - - 50 SPA17/CT22 - - - - - - - - 26 - - - - - - - 51 OYTES1/CT23 28 - 40 15 - 0 - 40 - 20 - - - - 0 - 52 MMA1a/CT25.1a - - 0 0 0 - - - - 40 26 - 0 - - 18 15 CAGE/CT26 - - - - - 89 - - - 100 - - - - - - 53 HOMTES85/CT28 - 35 0 10 - - - 19 - 28 36 32 - 0 - - 54 D40/CT29 - 20 - 13 - 0 - - - 41 - 36 27 - - - 55 HCA661/CT30 0 - - - - 0 0 29 - - 20 - - - - - 56 PLU-1/CT31 - - 86 - - - - - - - - - - - - - 27 LDHC/CT32 - - 35 15 - - - - - 47 44 42 - 37 57 - 18 MORC/CT33 - - 0 0 - - - - - 18 18 14 - 0 0 - 18 SGY-1/CT34 - - 20 0 - - - - - 12 25 57 - 12 0 - 18 SPO11/CT35 - - 0 0 - - - - - 0 6 0 - 0 0 - 18 TPX1/CT36 - - 15 0 - - - - - - 6 14 - 37 14 - 18 NYSAR35/CT37 42 - 23 0 8 - - - - 17 6 8 - - 0 8 57 FTHL17/CT38 22 - 14 0 0 - 10 - 0 25 0 - - 0 0 0 58 NXF2/CT39 19 - 0 11 12 - 5 - 0 15 55 - - 14 0 27 58 TAF7L/CT40 10 - 0 0 0 - 10 - 0 9 21 - - 0 0 12 58 TDRD1/CT41.1 28 - 37 0 10 - 22 - 5 5 0 - - 38 0 0 58 TEX15/CT42 21 - 0 0 20 - 11 - 0 21 27 - - 12 33 28 58 FATE/CT43 - - - 21 - 7 - 66 - 0 - - - - - - 19 TPTE/CT44 - - - 0 - 0 - 39 - 36 - - - - - - 19 aAbbreviations: Blad, bladder; Brn, brain; Brst, breast; Col, colon; Gas, gastric; H/N, head and neck; Leuk, leukemia; Lymph, lymphoma, NSCLC, non-small cell lung carcinoma; Mel, melanoma; Ov, ovarian; Pancr, pancreatic; Pros, prostate; Sarc, sarcoma; Ref, reference. bReference 59.
- Table 4
Normal tissue expressiona profiles of CT genes as determined by RT-PCRb.
CT Genes with Testis-Restricted mRNA Expression (µg cDNA product) BAGE/CT2.1 (0.02), MAGEE1/ CT10 (>2.0), CSAGE/CT24.1 (0.01), SPO11/CT35 (0.02), MAGEB1/CT3.1 (>2.0), SAGE/CT14 (0.02), MMA-1/CT25.1a (0.02), TPX1/CT36 (0.02), GAGE1/CT4.1 (>2.0), LIP1/CT17 (0.02), HOM-TES-85/CT28 (0.75), FTHL17/CT38 (>2.0), SSX2/CT5.2 (0.04), NA88/CT18 (0.01), LDHC/CT32 (0.04), TPTE/CT44 (0.08), BRDT/CT9 (0.02), CTAGE-1/CT21.1 (0.02), MORC/CT33 (0.06) CT Transcripts Detected in 2 or Fewer Non-gametogeic Tissues CT Transcript Tissue (µg cDNA product) MAGEA1/CT1.1 testis (>2.0), pancreas (0.02) NY-ESO-1/CT6.1 testis (>2.0), placenta (0.05), pancreas (0.02), liver (0.11) MAGEC1/CT7.1 testis (0.14), pancreas (0.09) SYCP1/CT8 testis (>2.0), pancreas (0.03) SPANXC/CT11.3 testis (2.0), placenta (0.10), pancreas (1.2), liver (0.19) HAGE/CT13 testis (0.02), pancreas (0.01) ADAM2/CT15 testis (0.03), pancreas (0.02) SGY-1/CT34 testis (1.87), ovary (0.01), pancreas (0.03), spleen (0.01) NY-SAR-35/CT37 testis (1.3), placenta (0.01), pancreas (0.02), liver (0.01) TDRD1/CT41.1 testis (0.08), pancreas (0.02), spleen (0.01) Differentially Expressedc CT Genes CT Transcript Tissue (µg cDNA product) XAGE-1a/CT12.1a testis (>2.0), placenta (0.03), pancreas (0.02), spleen (0.02), thymus (0.02), lung (>2.0), skeletal muscle (0.01) PAGE-5/CT16.1 testis (0.07), pancreas (0.01), spleen (0.01), kidney (0.02), liver (0.02) CAGE/CT26 testis (0.23), pancreas (0.03), leukocytes (0.01), brain (0.01) BORIS/CT27 testis (>2.0), pancreas (0.03), prostate (0.01), thymus (0.01), kidney (0.02) D40/CT29 testis (0.05), placenta (0.02), pancreas (0.01), thymus (0.02), colon (0.01), lung (0.01) NXF2/CT39 testis (>2.0), pancreas (0.02), prostate (0.02), brain (0.02), kidney (0.02), lung (0.03) TEX15/CT42 testis (>2.0), ovary (0.02), pancreas (0.07), spleen (0.02), liver (0.06), small intestine (0.06), prostate (0.01) FATE/CT43 testis (>2.0), ovary (0.01), thymus (0.06), heart (0.02), kidney (0.02), lung (0.02), skeletal muscle (0.02), pancreas (0.02) TAF7L/CT40 testis (0.15), placenta (0.03), pancreas (0.03), prostate (0.01), brain (0.01), kidney (0.03), liver (0.03), small intestine (0.02) Ubiquitously Expressed CT Genes (Figure 1) IL-13r alpha/CT19, SPA17/CT22, PLU-1/CT31, TSP50/CT20, OY-TES-1/CT23 aCommercially available, normalized cDNA (MTC panels, BD Biosciences, Palo Alto CA) derived from 13 non-gametogenic tissues (colon, leukocyte, prostate, spleen, thymus, brain, heart, kidney, liver, lung, skeletal muscle, pancreas and small intestine), as well as testis, ovary and placenta. b35 cycles of PCR amplification were performed using primer sequences and annealing temperatures described in primary publications. cDifferentially Expressed CT Transcripts were detected in 3-6 of the 13 non-gametogenic tissues analyzed.
Volume 4, Issue 1
