Article Figures & Data
Figures
- Figure 1
RT-PCR data showing high frequency of expression of non CT-X transcripts CTAGE1, HAGE/DDX43, CAGE/DDX53, Sp17 and PLU-1/JARID1B CT transcripts as opposed to low frequency of expression of CT-X transcripts GAGE1/2 and SSX2 in cell lines derived from haematological malignancies. Although the results were not assessed quantitatively, differential expression of transcripts between cell lines can clearly be seen.
- Figure 2
Graphical representation of the total number of CT transcripts expressed in individual cell lines.
- Table 1
Expression of CT transcripts in cell lines derived from DLBCL as assessed by RT-PCR.
- Table 2
Expression of CT transcripts in cell lines derived from non-DLBCL B-cell and T-cell malignancies as assessed by RT-PCR.
- Figure 3
Graphical representation of CT-X (black) and non CT-X (white) transcripts expressed in individual cell lines.
- Figure 4
Sp17 protein expression. (A) Anti-Sp17 antibody labels the cytoplasm of spermatogonia (weakly), and both cytoplasm and nuclei of spermatocytes and spermatozoa of normal testis. The intensity of the labelling increases as the cells increase in maturity. No staining was observed in normal tonsil sections. Sp17 expression was observed in scattered cells in DLBCL biopsies, DLBCL002, DLBCL005 and DLBCL003. A negative case is also illustrated (DLBCL013). (B) Western blotting showed expression of Sp17 protein (upper panel) in neoplastic B- and T-cell derived cell lines and testis. Heterogeneity of expression can also be observed between cell lines since the β-actin loading control (lower panel) suggests equal amounts of proteins were loaded. No Sp17 protein was detected in normal tonsil lysate. Samples: M, marker; To, normal tonsil lysate; Te, normal testis lysate; -, empty lane; DLBCL, lysates from DLBCL-derived cell lines; FL, lysates from follicular lymphoma-derived cell lines; BL, lysates from Burkitt's lymphoma-derived cell lines; HL, lysates from Hodgkin lymphoma-derived cell lines; MM, lysates from multiple myeloma-derived cell lines; T-ALL, lysates from T-acute lymphoblastic leukaemia/lymphoma-derived cell lines; A, lysate from anaplastic large cell lymphoma-derived cell line.
- Table 3
Primer sequences and annealing temperatures used in RT-PCR analyses of CT transcripts.
Additional Files
Supplementary Data
Supplementary Figure 1 (480 KB PDF file) - RT-PCR data showing normal tissue mRNA expression of CSAGE, CTAGE1, HAGE/DDX43, PLU-1/JARID1B, PRAME, Sp17 and CAGE/DDX53.
Supplementary Figure 2 (283 KB PDF file) - Western blotting of normal human tissue lysates with Sp17 antibody showed high levels of Sp17 protein in testis.
Supplementary Table 1 (119 KB PDF file) - Comparison of normal tissue mRNA expression data.
Entire supplemental data set (1.4 MB PDF file)
Volume 10, Issue 1
