Skip to main content
  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

AACR logo

  • Register
  • Log in
  • My Cart
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Cancer Immunology Essentials
    • Collections
      • COVID-19 & Cancer Resource Center
      • "Best of" Collection
      • Editors' Picks
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citation
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in
  • My Cart

Search

  • Advanced search
Cancer Immunology Research
Cancer Immunology Research
  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Cancer Immunology Essentials
    • Collections
      • COVID-19 & Cancer Resource Center
      • "Best of" Collection
      • Editors' Picks
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citation
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

Other Topics

Abstract B149: Real-time analysis of cell membrane protein and virus internalization using site-specific conjugation of protease-sensitive probes

Ross W. Cheloha, Zeyang Li, Djenet Bousbaine, Andrew Woodham, Priscillia Perrin, Jana Volaric and Hidde L. Ploegh
Ross W. Cheloha
Boston Children's Hospital, Harvard Medical School, Boston, MA; Massachusetts Institute of Technology, Cambridge, MA; Leiden University Medical Center, Leiden, The Netherlands; University of Groningen, Groningen, The Netherlands.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zeyang Li
Boston Children's Hospital, Harvard Medical School, Boston, MA; Massachusetts Institute of Technology, Cambridge, MA; Leiden University Medical Center, Leiden, The Netherlands; University of Groningen, Groningen, The Netherlands.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Djenet Bousbaine
Boston Children's Hospital, Harvard Medical School, Boston, MA; Massachusetts Institute of Technology, Cambridge, MA; Leiden University Medical Center, Leiden, The Netherlands; University of Groningen, Groningen, The Netherlands.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andrew Woodham
Boston Children's Hospital, Harvard Medical School, Boston, MA; Massachusetts Institute of Technology, Cambridge, MA; Leiden University Medical Center, Leiden, The Netherlands; University of Groningen, Groningen, The Netherlands.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Priscillia Perrin
Boston Children's Hospital, Harvard Medical School, Boston, MA; Massachusetts Institute of Technology, Cambridge, MA; Leiden University Medical Center, Leiden, The Netherlands; University of Groningen, Groningen, The Netherlands.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jana Volaric
Boston Children's Hospital, Harvard Medical School, Boston, MA; Massachusetts Institute of Technology, Cambridge, MA; Leiden University Medical Center, Leiden, The Netherlands; University of Groningen, Groningen, The Netherlands.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hidde L. Ploegh
Boston Children's Hospital, Harvard Medical School, Boston, MA; Massachusetts Institute of Technology, Cambridge, MA; Leiden University Medical Center, Leiden, The Netherlands; University of Groningen, Groningen, The Netherlands.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1158/2326-6074.CRICIMTEATIAACR18-B149 Published February 2019
  • Article
  • Info & Metrics
Loading
Abstracts: Fourth CRI-CIMT-EATI-AACR International Cancer Immunotherapy Conference: Translating Science into Survival; September 30 - October 3, 2018; New York, NY

Abstract

Reagents that label specific subsets of cells are broadly useful for the treatment of cancer and modulation of cells of the immune system. Labeling is often achieved through the identification and characterization of proteins specifically expressed on selected cells but not others. Knowledge of cell membrane protein routing dynamics can be leveraged to efficiently deliver cytotoxic or immune stimulating payloads. The most common method for monitoring internalization relies on labeling proteins with antibodies modified with a fluorophore or other tag that can also be used to report on whether the protein of interest has been internalized. This approach requires indirect methods, such as multiple rounds of cell staining, to differentiate extracellular protein from protein that has been internalized and recycled to the cell surface. Here we report a method for the characterization of protein internalization in real time through the sortase-mediated, site-specific labeling of single domain antibodies or viral proteins with a newly developed, cathepsin-sensitive quenched-fluorophore probe. This approach allows quantitative measurement of the movement of proteins into protease-containing endosomes in real time in live cells. This method revealed variation in the rate of internalization for different cell surface receptors and allowed for kinetic characterization of influenza virus internalization. These findings help to explain the utility of certain single domain antibody-antigen conjugates for inducing humoral immune responses. The tools and methods described here should be useful for the identification of proteins expressed on target cells that are ideal for antibody-mediated drug delivery or for promotion of specific types of immune responses.

Citation Format: Ross W. Cheloha, Zeyang Li, Djenet Bousbaine, Andrew Woodham, Priscillia Perrin, Jana Volaric, Hidde L. Ploegh. Real-time analysis of cell membrane protein and virus internalization using site-specific conjugation of protease-sensitive probes [abstract]. In: Proceedings of the Fourth CRI-CIMT-EATI-AACR International Cancer Immunotherapy Conference: Translating Science into Survival; Sept 30-Oct 3, 2018; New York, NY. Philadelphia (PA): AACR; Cancer Immunol Res 2019;7(2 Suppl):Abstract nr B149.

  • ©2019 American Association for Cancer Research.
Previous
Back to top
Cancer Immunology Research: 7 (2 Supplement)
February 2019
Volume 7, Issue 2 Supplement
  • Table of Contents

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Immunology Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Abstract B149: Real-time analysis of cell membrane protein and virus internalization using site-specific conjugation of protease-sensitive probes
(Your Name) has forwarded a page to you from Cancer Immunology Research
(Your Name) thought you would be interested in this article in Cancer Immunology Research.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract B149: Real-time analysis of cell membrane protein and virus internalization using site-specific conjugation of protease-sensitive probes
Ross W. Cheloha, Zeyang Li, Djenet Bousbaine, Andrew Woodham, Priscillia Perrin, Jana Volaric and Hidde L. Ploegh
Cancer Immunol Res February 1 2019 (7) (2 Supplement) B149; DOI: 10.1158/2326-6074.CRICIMTEATIAACR18-B149

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Abstract B149: Real-time analysis of cell membrane protein and virus internalization using site-specific conjugation of protease-sensitive probes
Ross W. Cheloha, Zeyang Li, Djenet Bousbaine, Andrew Woodham, Priscillia Perrin, Jana Volaric and Hidde L. Ploegh
Cancer Immunol Res February 1 2019 (7) (2 Supplement) B149; DOI: 10.1158/2326-6074.CRICIMTEATIAACR18-B149
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

Other Topics

  • Abstract B170: Therapeutic implications of altered epigenetics and DNA damage responses in IDH2-mutated hematologic diseases
  • Abstract B200: Single-cell RNA-sequencing (ScRNA-seq) reveals broad heterogeneity among CD8 T-cells during chronic viral infection and identifies a critical role for CD4 help in promoting the differentiation of a potent cytotoxic CD8 T-cell subset
  • Abstract B197: Translational control in macrophages during inflammatory response
Show more Other Topics

Other Topics: Poster Presentations - Proffered Abstracts

  • Abstract B170: Therapeutic implications of altered epigenetics and DNA damage responses in IDH2-mutated hematologic diseases
  • Abstract B200: Single-cell RNA-sequencing (ScRNA-seq) reveals broad heterogeneity among CD8 T-cells during chronic viral infection and identifies a critical role for CD4 help in promoting the differentiation of a potent cytotoxic CD8 T-cell subset
  • Abstract B197: Translational control in macrophages during inflammatory response
Show more Other Topics: Poster Presentations - Proffered Abstracts
  • Home
  • Alerts
  • Feedback
  • Privacy Policy
Facebook   Twitter   LinkedIn   YouTube   RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Cancer Immunology Essentials

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians

About Cancer Immunology Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2021 by the American Association for Cancer Research.

Cancer Immunology Research
eISSN: 2326-6074
ISSN: 2326-6066

Advertisement