Skip to main content
  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

AACR logo

  • Register
  • Log in
  • My Cart
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Cancer Immunology Essentials
    • Collections
      • COVID-19 & Cancer Resource Center
      • "Best of" Collection
      • Editors' Picks
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citation
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in
  • My Cart

Search

  • Advanced search
Cancer Immunology Research
Cancer Immunology Research
  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Cancer Immunology Essentials
    • Collections
      • COVID-19 & Cancer Resource Center
      • "Best of" Collection
      • Editors' Picks
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citation
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

Other Topics

Abstract A059: Combinatorial proteomic analysis of the receptor programmed cell death (PD)-1 uncovers new checkpoint modulators

Michael Peled and Adam Mor
Michael Peled
NYU School of Medicine, New York, NY.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Adam Mor
NYU School of Medicine, New York, NY.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1158/2326-6066.IMM2016-A059 Published November 2016
  • Article
  • Info & Metrics
Loading
Abstracts: Second CRI-CIMT-EATI-AACR International Cancer Immunotherapy Conference: Translating Science into Survival; September 25-28, 2016; New York, NY

Abstract

Programmed cell death (PD)-1 is one of the most important inhibitory checkpoints in T cells. Antibodies targeting PD-1 have elicited durable clinical responses in multiple tumor types and long-term remissions in some. Nevertheless, response to anti-PD-1 approaches is limited to a small fraction of patients and thus, a more complete understanding of this pathway should provide i) novel biomarkers for potential responders and ii) potential new therapeutic targets for refractory patients. We took an unbiased approach, exploiting high-resolution mass spectrometry, to uncover novel proteins that either interact with the cytoplasmic tail of PD-1 or are dephosphorylated downstream of the PD-1. We identified almost 20 proteins that interacted directly with the tail of PD-1, and approximately 100 proteins that their phosphorylation status was changed upon PD-1 signaling. Two of the more abundant proteins, EFHD2 and SH2D1A, were functionally analyzed for their contribution in mediating PD-1 inhibitory responses. Silencing EFHD2 resulted in abrogation of PD-1 inhibitory effect, including elimination of the ability of PD-1 signaling to inhibit cytokine secretion, cell proliferation, and cellular adhesion. Mechanistically, EFHD2 co-localized with PD-1 in the immunologic synapse, and was necessary for PD-1 clustering. In contrast, silencing SH2D1A augmented PD-1 function while over-expression of the same protein blocked PD-1 effects. We than discovered that SH2D1A physically competed with the phosphatase SHP2 for the same binding site on the tail of PD-1. Studying PD-1 interactome and phosphoproteome has thus identified new proteins that can modulate PD-1 function in opposite ways. These proteins should be further explored as potential targets in cancer.

Citation Format: Michael Peled, Adam Mor. Combinatorial proteomic analysis of the receptor programmed cell death (PD)-1 uncovers new checkpoint modulators [abstract]. In: Proceedings of the Second CRI-CIMT-EATI-AACR International Cancer Immunotherapy Conference: Translating Science into Survival; 2016 Sept 25-28; New York, NY. Philadelphia (PA): AACR; Cancer Immunol Res 2016;4(11 Suppl):Abstract nr A059.

  • ©2016 American Association for Cancer Research.
Previous
Back to top
Cancer Immunology Research: 4 (11 Supplement)
November 2016
Volume 4, Issue 11 Supplement
  • Table of Contents

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Immunology Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Abstract A059: Combinatorial proteomic analysis of the receptor programmed cell death (PD)-1 uncovers new checkpoint modulators
(Your Name) has forwarded a page to you from Cancer Immunology Research
(Your Name) thought you would be interested in this article in Cancer Immunology Research.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract A059: Combinatorial proteomic analysis of the receptor programmed cell death (PD)-1 uncovers new checkpoint modulators
Michael Peled and Adam Mor
Cancer Immunol Res November 1 2016 (4) (11 Supplement) A059; DOI: 10.1158/2326-6066.IMM2016-A059

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Abstract A059: Combinatorial proteomic analysis of the receptor programmed cell death (PD)-1 uncovers new checkpoint modulators
Michael Peled and Adam Mor
Cancer Immunol Res November 1 2016 (4) (11 Supplement) A059; DOI: 10.1158/2326-6066.IMM2016-A059
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

Other Topics

  • Abstract B170: Therapeutic implications of altered epigenetics and DNA damage responses in IDH2-mutated hematologic diseases
  • Abstract B200: Single-cell RNA-sequencing (ScRNA-seq) reveals broad heterogeneity among CD8 T-cells during chronic viral infection and identifies a critical role for CD4 help in promoting the differentiation of a potent cytotoxic CD8 T-cell subset
  • Abstract B197: Translational control in macrophages during inflammatory response
Show more Other Topics

Other Topics: Poster Presentations - Proffered Abstracts

  • Abstract B170: Therapeutic implications of altered epigenetics and DNA damage responses in IDH2-mutated hematologic diseases
  • Abstract B200: Single-cell RNA-sequencing (ScRNA-seq) reveals broad heterogeneity among CD8 T-cells during chronic viral infection and identifies a critical role for CD4 help in promoting the differentiation of a potent cytotoxic CD8 T-cell subset
  • Abstract B197: Translational control in macrophages during inflammatory response
Show more Other Topics: Poster Presentations - Proffered Abstracts
  • Home
  • Alerts
  • Feedback
  • Privacy Policy
Facebook   Twitter   LinkedIn   YouTube   RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Cancer Immunology Essentials

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians

About Cancer Immunology Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2021 by the American Association for Cancer Research.

Cancer Immunology Research
eISSN: 2326-6074
ISSN: 2326-6066

Advertisement