Table 1.

TCRα/β sequences of HLA–KLL tetramer+ clones from four MCC patients

Alpha chainBeta chainFunctional assays
PtV geneCDR3 regionJ geneV geneCDR3 regionJ geneEC50 (ng/mL peptide)bEC50 (ng/uL DNA)Response to MS-1Mutant Tet Binding
w830 clonotypes
1TRAV24*01CAFNTDKLIFTRAJ34*01TRBV12-4*01CASSLAGFRFFTRBJ2-1*014.6NoLower
0.4
2TRAV38-1*01CALTSGSRLTFTRAJ27*01TRBV19*01CASSIMLYSNQPQHFTRBJ1-5*0112140NoEqual
1.6
3TRAV38-1*01CAYPSTDKLIFTRAJ34*01TRBV19*01CASSILGASNQPQHFTRBJ1-5*01270Equal
4TRAV12-1*01CVLNNNDMRFTRAJ43*01TRBV19*01CASSILGASNQPQHFTRBJ1-5*011.1NoEqual
5TRAV12-1*01CVVNANDMRFTRAJ43*01TRBV10-3*01CAIRARDQNTGELFFTRBJ2-2*015.1NoEqual
0.89
w683 clonotypes
1TRAV12-1*01CVVALYSGGGADGLTFTRAJ45*01TRBV6-5*01CASRSQNYYGYTFTRBJ1-2*011.9NoLower
0.36
0.26
2TRAV12-1*01CVLNNNDMRFTRAJ43*01TRBV6-5*01CASRSQNYYGYTFTRBJ1-2*010.21NoLower
w678 clonotypes
1TRAV12-1*01CVLNNNDMRFTRAJ43*01TRBV10-3*01CAIRQFDANTGELFFTRBJ2-2*010.430.32YesEqual
0.50
0.47
1.5UNKNOWNaTRBV10-3*01CAIRQFDANTGELFFTRBJ2-2*010.84YesEqual
2TRAV38-1*01CAFRVSHDMRFTRAJ43*01TRBV19*01CASSIIAGSSYNEQFFTRBJ2-1*010.017YesLower
0.012
3TRAV12-1*01CVVATYSGGGADGLTFTRAJ45*01TRBV19*01CASSIIAGSSYNEQFFTRBJ2-1*010.0221.0YesEqual
0.013
4TRAV12-1*01CVVATYSGGGADGLTFTRAJ45*01TRBV10-2*01CASSSGNPSTDTQYFTRBJ2-3*010.0094YesEqual
0.028
5TRAV38-1*01CAFRVSHDMRFTRAJ43*01TRBV10-2*01CASSSGNPSTDTQYFTRBJ2-3*010.11YesLower
0.054
UNKNOWNaUNKNOWNa0.060YesEqual
0.058
w876 clonotypes
1TRAV12-1*01CVVGEYSGGGADGLTFTRAJ45*01TRBV28*01CAIRAGASYNEQFFTRBJ2-1*010.315.6Lower
2TRAV12-1*01CVVTEYSGGGADGLTFTRAJ45*01TRBV10-3*01CASRGQNTGELFFTRBJ2-2*011.2NoLower
3TRAV19*01CALGGGTFTSGTYKYIFTRAJ40*01TRBV9*02CASSVEDYTGELFFTRBJ2-2*010.1211NoEqual
4TRAV12-1*01CVVYTGYSGGGADGLTFTRAJ45*01TRBV10-2*01CASSVLNTGELFFTRBJ2-2*010.3114NoEqual
5TRAV38-1*01CAYNQGGKLIFTRAJ23*01TRBV10-2*01CASSVLNTGELFFTRBJ2-2*010.11NoEqual
6TRAV12-1*01CVVPLYSSASKIIFTRAJ3*01TRBV6-1*01CASSDTPDLNTEAFFTRBJ1-1*010.015NoLower
0.035
7TRAV12-1*01CVLNNNDRFTRAJ43*01TRBV6-1*01CASSDTPDLNTEAFFTRBJ1-1*010.143.6NoLower
8TRAV12-1*01CVVYASKIIFTRAJ3*01TRBV6-1*01CASSDTPDLNTEAFFTRBJ1-1*014.2Lower
9TRAV12-1*01CVGNNNDMRFTRAJ43*01TRBV10-3*01CAISARDQNTGELFFTRBJ2-2*010.12NoLower
0.24
10TRAV12-1*01CVVYGSSNTGKLIFTRAJ37*02TRBV10-3*01CAIRRQDQNTGELFFTRBJ2-2*010.70NoLower
11TRAV12-1*01CVVYTGYSGGGADGLTFTRAJ45*01TRBV10-3*01CAIHEGDSNTGELFFTRBJ2-2*01Equal
12TRAV3*01CAVRDNSGTYKYIFTRAJ40*01TRBV7-2*04CASSLAGLAGTDTQYFTRBJ2-3*01Lower
13TRAV12-1*01CVVTDTSGGGADGLTFTRAJ45*01TRBV7-2*04CASSLAGLAGTDTQYFTRBJ2-3*013.9
14TRAV12-1*01CVVPSAGKSTFTRAJ27*01TRBV2*03CASSEFAGQETQYFTRBJ2-5*015.4Lower
15UNKNOWNaTRBV6-5*01CASRASNTYGYTFTRBJ1-2*0180No
16TRAV38-1*01CAYNQGGKLIFTRAJ23*01TRBV19*01CASSTLSGTHNEQFFTRBJ2-1*010.12NoLower
17TRAV12-1*01CVVYGSSNTGKLIFTRAJ37*02TRBV7-2*04CASSLAGLANNEQFFTRBJ2-1*01Lower
18TRAV14CAMREAQSGGYQKVTFTRAJ13*01TRBV12-4*01CASSFGSGTKDTQYFTRBJ2-3*01Equal
19TRAV12-1*01CVVYTGYSGGGADGLTFTRAJ45*01TRBV10-2*01CASSGQNTGELFFTRBJ2-2*010.92NoLower
20TRAV10*01CVVSAGINGADGLTFTRAJ45*01TRBV12-4*01CASSPWDEQFFTRBJ2-1*01Lower
21TRAV3*01CAVRDNSGTYKYIFTRAJ40*01TRBV13*02CASSSGTSGGLTYNEQFFTRBJ2-1*01
22UNKNOWNaTRBV13*02CASSSRTKAYEQYFTRBJ2-7*01
23TRAV12-3*01CAMSVAQGGSEKLVFTRAJ57*01TRBV6-6*01CASSYQIGLSYEQYFTRBJ2-7*01
24UNKNOWNaTRBV28*01CASSFDSKGSNTGELFFTRBJ2-2*01
25TRAV27*01CAGDQGGSEKLVFTRAJ57*01TRBV16*01CASSQLRTGDEYEQYFTRBJ2-7*01
26TRAV12-1*01CVVYTGYSGGGADGLTFTRAJ45*01TRBV13*02CASSSGTSGGLNYNEQFFTRBJ2-1*01
27TRAV3*01CALTGYSTLTFTRAJ11*01TRBV19*01CASRSQLAVLNEQFFTRBJ2-1*01
28UNKNOWNaTRBV28*01CASRGGSSYNEQFFTRBJ2-1*01
29TRAV12-1*01CVVPLYSSASKIIFTRAJ3*01TRBV10-2*01CASSVLNTGELFFTRBJ2-2*010.123.3NoEqual
0.16
30UNKNOWNaTRBV10-3*01CATRDINTGELFFTRBJ2-2*01
31UNKNOWNaTRBV6-1*01CASSDTPDLNTEAFFTRBJ1-1*0124
  • aSelect TRA or TRB sequences were unresolved with next-generation sequencing.

  • bMultiple values represent data from replicate experiments.