Table 1.

PD-L1 expression in melanoma cell lines as a result of HDAC inhibition

Cell lineTreatmentPD-L1 MFIChange over DMSO (%)
WM983AAutofluorescence328NA
DMSO386NA
LBH589 (10 nmol/L)835216
MS275 (500 nmol/L)546141
MGCD0103 (500 nmol/L)1,151298
WM793Autofluorescence1,568NA
DMSO1,744NA
LBH589 (10 nmol/L)2,361135
MS275 (500 nmol/L)2,730157
MGCD0103 (500 nmol/L)2,108121
B78H1Autofluorescence651NA
DMSO9,044NA
LBH589 (10 nmol/L)13,839153
MS275 (500 nmol/L)13,051144
MGCD0103 (500 nmol/L)18,364203
SkMel21Autofluorescence1,404NA
DMSO3,686NA
LBH589 (10 nmol/L)10,616288
MS275 (500 nmol/L)16,508448
MGCD0103 (500 nmol/L)7,171195
WM35Autofluorescence698NA
DMSO1,095NA
LBH589 (10 nmol/L)1,654151
MS275 (500 nmol/L)1,693155
MGCD0103 (500 nmol/L)1,261115
WM1366Autofluorescence648NA
DMSO4,788NA
LBH589 (10 nmol/L)8,184171
MS275 (500 nmol/L)6,999146
MGCD0103 (500 nmol/L)10,169212
B16Autofluorescence206NA
DMSO1,297NA
LBH589 (10 nmol/L)4,139319
MS275 (500 nmol/L)2,480191
MGCD0103 (500 nmol/L)7,350567
624Autofluorescence1,337NA
DMSO4,241NA
LBH589 (10 nmol/L)6,211146
MS275 (500 nmol/L)6,446152
MGCD0103 (500 nmol/L)7,142168
888Autofluorescence1,027NA
DMSO2,097NA
LBH589 (10 nmol/L)2,999143
MS275 (500 nmol/L)3,608172
MGCD0103 (500 nmol/L)3,281156

NOTE: Mean fluorescence intensity (MFI) and the percentage (%) of change over DMSO control are shown for various melanoma cell lines treated for 72 hours with LBH589, MS275, and MGCD0103 HDAC inhibitors, at indicated concentrations.

Abbreviation: NA, not applicable.