Table 4.

Relationship of TIL subsets to tumor and TIL PD-L1 expression

TumorTILs
All SpecimensPD-L1(−)PD-L1+PD-L1(−)PD-L1+
n = 39an = 15n = 24Pbn = 11n = 28Pb
Intratumoral CD4:CD8, n (%)
 CD4 ≥ CD816 (41)12 (80)4 (17)<0.0019 (82)7 (25)0.003
 CD8 > CD423 (59)3 (20)20 (83)2 (18)21 (75)
Peritumoral CD4:CD8, n (%)
 CD4 ≥ CD835 (90)15 (100)20 (83)0.14611 (100)24 (86)0.309
 CD8 > CD44 (10)0 (0)4 (17)0 (0)4 (14)
All specimensPD-L1(−)PD-L1+PD-L1(−)PD-L1+
n = 40an = 15n = 25Pbn = 11n = 29Pb
CD8 Intratumoral, n (%)
 ≤60/HPF31 (78)15 (100)16 (64)0.01511 (100)20 (69)0.043
 >60/HPF9 (22)0 (0)9 (36)0 (0)9 (31)
CD8 Peritumoral, n (%)
 ≤60/HPF14 (35)12 (80)2 (8)<0.00110 (91)4 (14)<0.001
 >60/HPF26 (65)3 (20)23 (92)1 (9)25 (86)
  • aThirty-nine specimens had TILs present and tissue available for CD4 staining. Forty specimens had TILs present and material for CD8 staining.

  • bFisher exact test.