MicroRNAs are endogenous noncoding RNAs that play important roles in a wide variety of biological processes such as apoptosis, development, aging and tumorigenesis. The BCL6 (B-cell lymphoma 6) transcriptional repressor has emerged as a critical therapeutic target in diffuse large B-cell lymphomas (DLBCL), but the mechanisms regulating BCL6 are still unclear. In the current study we screened the microRNA expression profiles in DLBCL specimens and cell lines by qRT-PCR, and found that the expression of miR-144 was significantly downregulated in DLBCL tissues and cell lines and negatively correlated with BCL6 expression. We further demonstrated that BCL6 was the direct target gene of miR-144, and miR-144 suppressed the expression of BCL6 via binding the 3'UTR of BCL6 mRNA. Biologically, forced expression of miR-144 significantly attenuated cell proliferation and invasion of OCI-Ly3 cells in vitro, and the tumor-suppressor effect of miR-144 was also confirmed using a xenograft mouse model in vivo. Taken together, our results revealed that miR-144 regulates BCL6 in DLBCL and provided rationales for developing strategies that target miR-144 as a therapeutic intervention for DLBCL.
- Received July 4, 2015.
- Revision received November 17, 2015.
- Accepted December 17, 2015.
- Copyright © 2016, American Association for Cancer Research.