Adaptive co-evolution has formed a complex network of inextricable bonds between the resident microbes and the mammalian host. These symbiotic microbes are now believed to have critical impacts on human health. One of the most common gut symbionts, Bacteroides fragilis, produces an outersurface polysaccharide (PSA) that possesses immunomodulatory effects on the host. We adapted a metabolic labeling approach to study the microbiota communication with the host in real-time. We labeled PSA and detected B. fragilis in vivo associated with immune cells and organs. We extended this labeling approach to commensals from diverse phyla in order to study their effects on the host. This method has the potential to enhance our understanding of microbiota colonization as well as to define a set of characteristics of protective cells whose stimulation by previously undiscovered commensal-derived molecules may represent an important new approach to the treatment and prevention of diseases.
Citation Format: Naama Geva-Zatorsky, David Alvarez, Jason E. Hudak, Nicola C. Reading Mans, Deniz Erturk-Hasdemir, Suryasarathi Dasgupta, Ulrich von Andrian, Dennis Kasper. Gut microbiota-host immunomodulatory interactions [abstract]. In: Proceedings of the Second CRI-CIMT-EATI-AACR International Cancer Immunotherapy Conference: Translating Science into Survival; 2016 Sept 25-28; New York, NY. Philadelphia (PA): AACR; Cancer Immunol Res 2016;4(11 Suppl):Abstract nr IA18.
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