Microbiota generates unique metabolites to modulate host immunity associated with pathogen resistance and inflammatory diseases, but the specific factors and mechanisms of actions are not well understood. To the end, the Hang and Mucida laboratories have recently identified a secreted peptidoglycan hydrolase, SagA, from E. faecium that generates uniquely active muropeptides that enhance host epithelial barrier integrity and resistance to enteric pathogens in worms and mice. The molecular targets of SagA-generated muropeptides are still unknown and will be addressed using chemical biology and proteomics approaches. Here, we report progress towards the synthesis of SagA-generated muropeptide chemical probes for target identification of specific protein receptors and signaling pathways. Elucidation of SagA-muropeptide protein targets should reveal new pathways to modulate host immunity and inflammation-associated diseases and cancer.
Citation Format: Yen-Chih Wang. Chemical biology of microbial metabolite targets in host immunity [abstract]. In: Proceedings of the Second CRI-CIMT-EATI-AACR International Cancer Immunotherapy Conference: Translating Science into Survival; 2016 Sept 25-28; New York, NY. Philadelphia (PA): AACR; Cancer Immunol Res 2016;4(11 Suppl):Abstract nr A081.
- ©2016 American Association for Cancer Research.